NS-398, a selective cyclooxygenase-2 inhibitor, can down-regulate the expression of P-glycoprotein in the gastric cancer cell
- VernacularTitle:选择性环氧合酶-2抑制剂NS-398抑制胃癌细胞P-糖蛋白表达
- Author:
Gen LIN
;
Yuli CHEN
;
Zhizhe CHEN
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Cyclooxygenase inhibitor;
P-glycoprotein;
Multiple drug resistance
- From:
Chinese Journal of Digestion
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of NS-398, a selective cyclooxygenase-2 (COX-2) inhibitor, on the expression of P-glycoprotein(P-gp) in a gastric cancer cell line SGC-7901. Methods SGC-7901 cells were treated with NS-398 in different concentrations (0, 10 ?mol/L and 100 ?mol/L) respectively. Prostaglandin E2(PGE2) was detected by ELISA. Twenty four hs and 48 hs later, the expression of mdr1 mRNA were detected by RT-PCR. P-gp protein expression in SGC-7901 cells was detected by immunocytochemical technique after NS-398 treatment.Results NS-398 can inhibit the expression of PGE2 in a dose-dependent manner(P
