HPLC fingerprint of Rumex gmelini from different habitats
- VernacularTitle:不同产地毛脉酸模药材HPLC指纹图谱研究
- Author:
Zhenyue WANG
;
Yueming ZUO
;
Yihua KANG
;
Ruiming LI
;
Honghua CUI
- Publication Type:Journal Article
- Keywords:
Rumex gmelini Turcz.;
fingerprint;
HPLC;
gradient elution
- From:
Chinese Traditional and Herbal Drugs
1994;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the HPLC fingerprint of Rumex gmelini from different habitats by RP-HPLC (DAD). Methods In this paper, 12 different samples were studied. Separation was performed on an Planetsil C_ 18 column, with mobile phase consisting of methanol and 0.1% phosphoric acid-water and with gradient elution at the flow rate of 1.0 mL/min. The UV detection wavelength was 254 nm, column temperature was 35 ℃, and the analysis time was 50 min. Results The results showed that this method has a good repeatability and the ratio of common peaksarea of different samples had some difference. Conclusion This method can be used to establish the chromatographic fingerprint of R. gmelini with high specificity.
