Comparison of Effects of Radix Astragali and Triperygium Glucosides on Glomerular Mesangial Cells Proliferation and Interleukin-6 Secretion
- VernacularTitle:黄芪和雷公藤多甙对大鼠肾小球系膜细胞增殖及分泌IL-6影响的比较
- Author:
Kun BAO
;
Wei MAO
;
Yi PANG
;
Dan ZHONG
- Publication Type:Journal Article
- Keywords:
RADIX ASTRAGALI/pharmacology;
TRIPERYGIUM GLUCOSIDES/pharmacology;
RENAL FAILURE, CHRONIC/TCD therapy;
CELL CULTURE
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2000;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To compare the effects of Radix Astragali (RA) and triperygium glucosides (TG) on glomerular mesangial cells (GMC) proliferation and interleukin-6 (IL-6) secretion. [Methods] GMC were cultured with in-vitro cell culture technology. Serum containing RA and serum containing TG were prepared with serum pharmacological methods. GMC proliferation was detected by methylthiazolyltetrazolium (MTT) assay and IL-6 level by enzyme-linked immunosorbent assay (ELISA) to evaluate the effect of low-, moderate- and high-dosages of RA-containing serum and TG-containing serum under the condition of GMC over-proliferation. [ Results] Different dosages of RA-containing serum and TG-containing serum inhibited GMC proliferation and IL-6 secretion in a dose-effect manner, and the inhibitory effect of high- and moderate-dosage TG and high-dosage RA was stronger. [Conclusion] Under the condition of in-vitro GMC over-proliferation, RA has the similar inhibitory effects on GMC proliferation and IL-6 secretion with TG. The combined treatment of RA and TG can reduce the dose of TG and probably has an action in increasing effect and reducing toxicity.
