Content Determination of Ferulic Acid and Salvianolic Acid B in Yixin Tablets by HPLC
- VernacularTitle:高效液相色谱法测定益心片中阿魏酸和丹酚酸B的含量
- Author:
Fengxia XIAO
;
Fengyun WANG
;
Huiyan FU
- Publication Type:Journal Article
- Keywords:
YIXIN TABLETS /chemistry;
FERULIC ACID/analysis;
SALVIANOLIC ACID B/analysis;
CHROMATOGRAPHY, HIGH PRESSURE LIQUID
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2000;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To establish a method for the determination of ferulic acid and salvianolic acid B contents in Yixin Tablets. [Methods] High pressure liquid chromatography (HPLC) was adopted. The chromatographic conditions were: 100-fold methanol as solvent, Bonification for 30min, gradient elution on C18 comlumn with acetonitrile and phosphoric acid as mobile phase, the detection wavelength being 320nm. [Results] Ferulic acid had a good linearity in the range of 0.024 32 - 0.729 60 ?g and r=0.999 9; salvianolic acid B had a good linearity in the range of 0.416 8-12.504 0?g and r = 0.9999; the average recovery rate was 100.95% in ferulic acid and 99.35% in salvianolic acid B respectively. [Conclusion] This method is simple, accurate and with good reproducibility.
