Methods of Avoiding Hemolysis in Preparing Serum Complements of Guinea Pigs
- VernacularTitle:制备豚鼠补体时避免溶血发生的方法学探讨
- Author:
Liuying CAO
;
Ruiyan LIANG
;
Huaxin PAN
;
Lian ZHOU
- Publication Type:Journal Article
- Keywords:
COMPLEMENTS/production & preparation;
HEMOLYSIS
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2000;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To explore the methods of avoiding hemolysis in preparing serum complements of guinea pigs. [Methods] In method 1, the serum of 8 guinea pigs was fixed first and then sheep red blood cells (SRBC) were added to the mixed serum for absorption. In method 2, SRBC was added to the serum of 8 guinea pigs respectively for absorption first and then the serum containing SRBC was mixed. After the experiment, macroscopic examination was carried out to observe the hemolysis and the optical density of the two kinds of mixture was detected at the wavelength of 540 nm. [ Results] In the serum complements of guinea pigs prepared by method 1, macroscopic hemolysis occurred; in the serum complements prepared by method 2, macroscopic hemolysis was not obvious and the optical density of the mixture was lower than that by method 1 ( P
