Effect of Aloe Polysaccharides on Thymocytic Apoptosis and Cell Cycle in ? - ray Irradiated Mice
- VernacularTitle:中华芦荟多糖对辐射小鼠胸腺细胞凋亡及细胞周期的影响
- Author:
Zongwei WANG
;
Anping YANG
;
Qingguang WU
;
Yina WU
;
Zhaosheng HUANG
;
Qiaomei LI
- Publication Type:Journal Article
- Keywords:
Aloe Polysaccharides;
? - ray irradiation;
Thymocytic apoptosis;
Cell cycle
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Aloe polysaccharides (AP) on the thymocytic apoptosis and cell cycle in ? - ray irradiated mice. Methods Single- cell thymocytes suspension was sampled at different time points to observe the thymocytic apoptosis and cell cycle by flow cytometry. DNA ladders were tested by 1.8 % agarose gel electrophoresis. Transmission electron microscopy was used to examine the ultrastructure of thymocytes. Results Pre- treating with AP (50 mg/kg,ip) 30 min before irradiation could significantly decrease the percentages of apoptotic thymocytes in? - ray irradiated mice 4 h, 8 h and 12 h after irradiation, increase the percentage of thymocytes at G0/G1 phase and reduce the percentage of thymocytes at G2/M phase. It could also lessen the DNA ladders and reduce the number of apoptotic bodies. Conclusion The protective effects of AP on the thymocytes in ? - ray irradiated mice is related with the alleviation of the disorder of cell cycle and the inhibition of the apoptosis of thymocytes.
