Determination of Protopine in Rhizoma Corydalis Decumbentis Capsule by HPLC
- VernacularTitle:HPLC法测定夏天无胶囊中原阿片碱的含量
- Author:
Xiangyang LI
;
Wanqian TU
- Publication Type:Journal Article
- Keywords:
Rhizoma Corydalis Decumbentis Capsules;
Protopine;
HPLC;
Content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a HPLC method for the determination of protopine in Rhizoma Corydalis Decumbentis Capsules. Methods A HPLC was performed on a Hypersil ODS column( 4.6? 250 mm, 5 ? m) . The mobile phase was methanol - 0.1 % water solution of triethylamine (65∶ 35). Determination wavelength was 290 nm and flow rate was 1.0 mL/min. The theoretical plates should over 4000 according to the content of protopine. Results A good linearity was shown in the range of 0.3168～ 1.5840 ? g, r=0.9996. The average recovery was 100.6 % , RSD=2.68 % . Conclusion The method is simple, effective and can be used for the quality control of Rhizoma Corydalis Decumbentis Capsules.
