The role of the expression of a proliferation-inducing ligand and its receptors in the gastric carcinogenesis
- VernacularTitle:胃黏膜癌变过程中增殖诱导配体及其受体表达水平分析
- Author:
Zhenbiao MAO
;
Donglei ZHANG
;
Jian SHI
- Publication Type:Journal Article
- Keywords:
Gastric cancer;
A proliferation-inducing ligand;
B cell maturation antigen;
Transmembrane activator
- From:
Chinese Journal of Digestion
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of the expression of a proliferation-inducing ligand (APRIL) and its receptors in the gastric carcinogenesis. Methods The real-time fluorescence quantitative polymerase chain reaction (RFQ-PCR) was used to detect expression of APRIL and its receptors. Based on the standard curves, the quantitative levels of target genes in tissue samples were determined by using software, and the results were presented as the ratios of mRNA levels of target genes to ?2-microgluobulin(?2M). Results The detection linear range of RFQ-PCR was 101-109 pg/ml and the coefficient of variation values for both intra-experimental and inter-experimental reproducibility ranged 6.52% - 12.02% and 8.76% - 14.16%, respectively.The expression levels of APRIL in the tissue of intestinal metaplasia , dysplasia and gastric cancer were significantly higher than those in normal tissue(P0.05 , respectively). Conclusions The present study indicated that RFQ-PCR had satisfied sensitivity and reproducibility in quantitative measurement of APRIL and its receptors. APRIL may play an important role in the development and progress of gastric cancer and could be established as a target molecule for early diagnosis and anti-cancer therapy. Besides, there maybe some unknown receptors of APRIL expressed on tumor tissue.
