Effect of polycystic ovary-like environment on E_2 and P levels in human granulosa cells in vitro
- VernacularTitle:类PCOS激素环境对颗粒细胞E_2、P分泌功能的影响
- Author:
Yan QIU
- Publication Type:Journal Article
- Keywords:
Polycystic ovary-like environment;
Granulosa cells;
Estradiol;
Progestrone
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of polycystic ovary-like environment on secretion of E_2 and P from human granulosa luteal cells in vitro.Methods:According to different additives granulosa cells cultured in vitro which were retrieved from infertility women with normal ovulation who were undergoing in vitro fertilization-embryo transfer(IVF-ET),were divided into four groups:A (androstenedione) group,LH group,INS (insulin) group and A+LH+INS group.Each group was subdivided into polycystic ovary-like environment group with higher levels of the additives and physiological environment group with lower levels of the additives.Levels of E_2 and P in medium were detected separately.Results:Levels of E_2 and P in polycystic ovary-like environment were significantly decreased in A group and A+LH+INS group,and significantly increased in LH group and INS group (P
