In vitro study on synergism of ECV304-SKOV3 and L_(929)-H_(22) cells in tumor angiogenesis
- VernacularTitle:ECV304-SKOV3和L_(929)-H_(22)细胞协同形成血管样结构的体外研究
- Author:
Hongmei ZHU
- Publication Type:Journal Article
- Keywords:
Tumor;
Fibroblasts;
Endothelial cell;
Angiogenesis;
Invasion
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the synergism of vascular endothelial cells and fibroblasts derived from tumors in the tumor angiogenesis after the establishment of the cell models.Methods:Reverse transcription polymerase chain reaction,two-well co-culture system were used to detect the tolomerase activity,and their abilities of migration and angiogenesis of ECV304-SKOV3 cells and the potential of L_(929)-H_(22) to promote angiogenesis and invasion of MDA-MB-231 cells,respectively,which were established as the cell models for vascular endothelial cells and fibroblasts derived from tumors in our laboratory before.Results:Tolomerase activity level of ECV304-SKOV3 cells was higher than that of ECV304 cells (0.778?0.011 vs 0.692?0.014,P
