Comparative study on the expressions of HGF and bFGF between fresh and preserved amniotic membranes
- VernacularTitle:新鲜羊膜与保存羊膜中HGF、bFGF表达的对照研究
- Author:
Jing LU
- Publication Type:Journal Article
- Keywords:
Fresh amniotic membrane;
Preserved amniotic membrane;
HGF;
bFGF;
Immunohistochemical
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the expressions of hepatocyte growth factor (HGF) and basic fibroblast growth factor (bFGF) in fresh and two preserved amniotic membranes (AM),and to explore their biological vitality.Methods:The amniotic membranes from the healthy puerperia with abdominal delivery were randomly divided into three groups:fresh group,DMEM/glycerol preserved group at -80℃ and glycerol preserved group at 4℃ (for 1 and 3 months).Immunohistochemical method was used to investigate the changes in the expressions of HGF and bFGF among the three groups.Results:HGF and bFGF were distributed in the epithelium of AMs mostly.The expressions of HGF in both preserved AMs for 1 and 3 months decreased significantly compared with fresh AM (P
