The effects of HBV infection on the ability of cellular immunity in human body
- VernacularTitle:HBV感染对人体T细胞免疫功能影响的研究
- Author:
Lian YANG
- Publication Type:Journal Article
- Keywords:
HBV;
T cellular immunity;
Flow cytometry;
Immunohistochemistry
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the probable mechanism of HBV effect on cellular immune system in human body by detecting the quantity and function of CD4~+T and CD8+T cells in peripheral blood and liver tisues.Methods:1)The quantity of CD4~+T and CD8~+T cells in peripheral blood was detected by flow cytometry(FCM) in 36 patients with hepatitis B and 20 cases of controls;2)The expressions of HBsAg and HBcAg were detected by streptavidin-peroxidase immunohistochemistry(SP).50 cases of positive HBsAg and(or)HBcAg were selected as HBV(+) group,and 10 cases of negative HBsAg and HBcAg in liver tissue and blood serum were selected as HBV(-) group.The expressions of CD4,CD8,CD25 in liver tissues were detected in the two groups.Results:1)The quantity of CD4~+T cells and the ratio of CD4~+T/CD8~+T in peripheral blood of patients was significantly lower than those in controls;the quantity of CD8~+T cells was considerably higher than that in controls.(2)T lymphoid cell infiltrates in the liver were most gathered in the lobules and the portal tract.The densities of CD8~+T cells were higher in lobules and portal tracts than CD4~+T cells.The quantity of CD8+T and CD4~+T cells in the liver tissues of HBV positive group showed significant difference from the liver tissues of HBV negative group(P~b
