Study on the effect of adiponectin on enzymatic activity of glucokinase and phosphoenolpyruvate carboxykinase in cultured rat's liver cells
- VernacularTitle:脂联素对大鼠肝细胞葡萄糖激酶与磷酸烯醇式丙酮酸羧激酶活性影响的研究
- Author:
Ming GONG
- Publication Type:Journal Article
- Keywords:
Adiponectin;
Glucokinase;
Phosphoenolpyruvate;
Carboxykinase;
Insulin resistance
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of adiponectin on enzymatic activity of glucokinase and phosphoenolpyruvate carboxykinase in cultured rat's liver cells.Methods:The BRL was used as cell model.The rat liver cells were incubated with adiponectin of three different concentrations (10?g/ml,30?g/ml and 50?g/ml).Controlled cells were incubated without adiponectin.Enzymatic activity of GK was investigated by using a standard G6P-coupled assay.Enzymatical activity of PEPCK was assessed by using a standard LDH-coupled assay.Results:Compared with the control group,treated group with adiponectin of all concentrations for 24h had no significant effect on enzymatic activity of glucokinase.Enzymatic activity of phosphoenolpyruvate carboxykinase was significantly decreased even in the lowest concentration of adiponectin,and along with increased in adiponectin concentration enzymatic activity of phosphoenolpyruvate carboxykinase decreased more significantly.Conclusion:Adiponectin has no significant effect on ezymatic activity of glucokinase.However adiponectin decreases enzymatic activity of phosphoenolpyruvate carboxykinase significantly.Through this effect,adiponectin decreases liver glucose production and improves insulin resistance.
