Simultaneous determination of hespefidin and baicalin in Qingqi Huatan Pill by HPLC
- VernacularTitle:RP-HPLC同时测定清气化痰丸中橙皮苷和黄芩苷的含量
- Author:
Yan YU
;
Haisheng JIAO
;
Lixing MA
- Publication Type:Journal Article
- Keywords:
hesperidin;
baicalin;
HPLC;
Qingqi Huatan Pill
- From:
Chinese Traditional Patent Medicine
1992;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a method for the simultaneous determination of hesperidin and baicalin in Qingqi Huatan Pill (Pericarpium Citri Reticulatae, Radix Scutellariae, etc.). METHODS: RP-HPLC was adopted with a mobile phase of acetonitrile-water-1% phosphoric acid (20∶80∶0.2), flow rate of 0.8 mL?min -1. The detection wavelength was at 280 nm. RESULTS:The linear range of hesperidin was 0.04 ?g-0.37 ?g, the average recovery was 97.5% and RSD was 1.0%. The linear range of baicalin was 0.13 ?g-1.19 ?g. The average recovery was 97.5%and RSD was 1.2%, respectively. CONCLUSION: This method is simple, practical, accurate and suitable for the quality control of Qingqi Huatan Pill.
