Determination of Baicalin in Huangqin Eye Drop by HPLC
- VernacularTitle:HPLC法测定黄芩滴眼液中黄芩苷的含量
- Author:
Weixian ZHAO
- Publication Type:Journal Article
- Keywords:
Huangqin Eye Drop;
Baicalin;
HPLC;
Content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of baicalin in Huangqin Eye Drops by HPLC. Methods A HPLC was performed on Shim- pack VP- ODS column(4.6 mm? 250 mm), a mixture of methanol- 0.2 % phosphoric acid (44 ∶ 55)was used as the mobile phase and the detection wavelength was 276nm. The flow rate was 1.0 mL/min with injection volume of 20 ? L and the column temperature was 35 ℃ .Results The linearity of baicalin was good in the range of 0.08～ 0.80 ? g (r=0.9997), and the average recovery was 100.10 % , with RSD 0.81 % . Conclusion This method was simple, sensitive and accurate , and can be used for quality control of Huangqin Eye Drops.
