Modulation and function of estrogen receptor isoforms ? and ? in endometrial carcinoma cells
- VernacularTitle:子宫内膜癌细胞雌激素受体亚型的调控和功能的初步研究
- Author:
Yan ZHANG
;
Qinping LIAO
;
Li YU
;
Jian ZHAO
;
Chunling CHEN
- Publication Type:Journal Article
- Keywords:
Endometrial neoplasms;
Receptors, estrogen;
Oligonucleotides, antisense
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore an efficient way to modulate the expression of estrogen receptor (ER) ? and ?, and to build up a model of endometrial cancer cell expressing predominantly one isoform of ER and to verify the roles of ER ? and ? in the tumorigenesis of endometrial cancer associated with estrogen and tamoxifen (TAM). Methods A series of oligodeoxyribonucleotides (ODN) against ? or ? regions of ER ? or ? were synthesized and tested in human endometrial cancer cell lines (Ishikawa) that express functional ER ? and ?. The expressions of two ER isoforms were detected by western blot using specific antibodies. Then we studied the change of Ishikawa proliferation in response to 17?-estradiol and TAM under the influence of antisense ODN. Results (1) Transfection with antisense ODN directed against the ER? and ER? could significantly inhibit target protein production. (2) 17?-estradiol could increase the proliferation of Ishikawa cells, but they lost the ability to proliferate in response to 17?-estradiol after transfected with ER? antisense ODN especially at hours 24, 48 and 72 ( P
