Determination of tripterine in Tripterygium wilfordii and its tablets by HPLC
- VernacularTitle:HPLC法测定雷公藤及其片剂中雷公藤红素
- Author:
Yan XIA
;
Wenyan WANG
;
Yanwen ZHANG
;
Jiangping HE
;
Wenyuan GAO
;
Hongquan DUAN
- Publication Type:Journal Article
- Keywords:
Tripterygium wilfordii Hook.f.;
tripterine;
HPLC
- From:
Chinese Traditional and Herbal Drugs
1994;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the content of tripterine in Tripterygium wilfordii and its tablets by HPLC.Methods An external standard method by HPLC with Zorbax C_(18)column as fixed phase and methanol-1% HAc(87∶13) as mobile phase was adopted.The detection wavelength was 425 nm and the flow rate was 1.0 mL/min.Results The linear range for tripterine was 40.96～204.8 ?g/mL(r=(0.999 6).) The average recovery of Chinese medicinal materials was 98.37% and RSD was 1.01%(n=9);the average recovery of preparation sample was 98.59% and RSD was 1.18%(n=9).Conclusion The method is simple and accurate,which can be adoptable for quantitative analysis of tripterine in the plants of Tripterygium L.
