IDENTIFICATION AND CHARACTERIZATION OF CD34~+ STEM CELLS OF HEMATOPOIETIC TUMOR CELL LINES
- VernacularTitle:血液系统肿瘤细胞系中CD34~+干细胞特性研究
- Author:
Lerong REN
;
Yuqin LIU
- Publication Type:Journal Article
- Keywords:
Tumor stem cells;
CD34;
FCM technology;
Cell cutured;
Fluorescence Staining;
Hematopoietic system;
Cell lines
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify and characterize the CD34~+ stem cells of hematopoietic tumor cell lines. Methods Taking CD34 molecule as a marker to identify if there are CD34~+ tumor cells in hematopoietic tumor cell lines(THP-1,MEL,K562,MOLT-4,RAJI,RPMI8226,SP2/0 and DCS) by fluorescence staining and FCM technology. We separated CD34~+ cells from K562 and MOLT-4 for further study.CD34~+ cells were cultured and observed their development.CD34~+ and CD34 +- cells were compared in their functional status(mRNA),cell cycles(G -0/G -1),their degree of differentiation(Id-1 expression) and their multi-drug resistance(gp expression). Results The percentages of CD34~+ cells were 6 ^2%(DCS),5.2%(MEL),1.4%(RAJI),2.5%(THP-1),2.1%(MOLT-4),3.1%(K562),0(SP2/0),and 0(RPMI8226) respectively.After culturing CD34 ++ cells isolated from K562 cells line could proliferate and the percentage of CD34 ++ reminded the same.CD34 ++ tumor cells contained much less RNA,more G0/G1 cells,expressed more Id-1 protein(less differentiated state) and possessed more P-gp(stronger multi-drug resistance).Conclusion There exists a very small portion of CD34 ++ stem cells among hematopoietic tumor cells and these cells are in a more primitive state.
