siRNA INHIBITS Her2/neu EXPRESSION AND CELL PROLIFERATION IN SK-BR-3 CELL LINE
- VernacularTitle:siRNA抑制SK-BR-3细胞株Her2/neu的表达及细胞增殖
- Author:
Jicheng LI
- Publication Type:Journal Article
- Keywords:
siRNA;
Her2/neu;
RNAi;
Breast cancer;
RT-PCR;
Flow cytometry
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Using siRNA to downregulate Her2 expression in Her2 positive breast cancer cell line SK-BR-3,to observe the influence of siRNA of cell proliferation and apoptosis. Methods Two siRNAs against the Her2 gene were designed and transfected into SK-BR-3 cells respectively, Her2 gene expression was measured using RT-PCR, Her2 protein expression was analysed using flow cytometer. Cell proliferation was measured with MTT assay, the apoptotic cells were stained with annexin-V-FITC and analysed by flow cytometry. Results 42.88%,49.27% silencing of Her2 mRNA expression were observed after transfecting with two siRNA respectively. The corresponding decrease of Her2 protein expression was confirmed by flow cytometric analysis. As a consequence, cell growth inhibition was observed,28.51% and 42.81% of cells apoptosis were observed after transfecting with two siRNA respectively.Conclusion These results demonstrated that siRNA against Her2 could effectively downregulate Her2 expression in SK-BR-3 cell line, inhibit cell growth and induce cell apoptosis.
