EFFECTS OF ESTROGEN ON ESTROGEN RECEPTOR ? AND ? IN RAT HEART
- VernacularTitle:雌激素对大鼠心脏雌激素受体?和?表达的影响
- Author:
Zongwen TIAN
- Publication Type:Journal Article
- Keywords:
Estrogen;
Estrogen receptor;
Ovariotomy;
RT-PCR;
Western blot;
Heart
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study estrogen receptor (ER) ? and ? expression in the newborn and adult rats,and effect of estrogen on ER? and ER? in ovariectomized rat heart.Methods By RT-PCR and Western blot analysis,changes in ER? and ER? in newborn and adult rat heart chambers were investigated,and subsequently,the effect of 17?-estradiol deficit and supplementation on ER? and ER?,and heart chamber wieght and ratio to body weight in ovariectomized rats were evaluated.Results ER? mRNA was lower in all heart chambers of newborn rats,but was elevated by 3-to 20-fold in the atria and ventricles of adult rats,these differences were efficiently translated into 67?kD ER? protein.Inversely,ER? mRNA expressed in the heart chambers of newborn rats was higher,and was dramatically decreased by 2-to 7-fold in all heart chambers of adult rats.In adult rats,ovariectomy decreased atrial ER? mRNA and ER? protein expression,and the atrial weight and atria/body weight ratio. Treatment with 17?-estradiol in ovariectomized rats reversed these changes;meanwhile these changes showed the dose-dependent relation to some extent along with the increment of 17?-estradiol dosage,but ovariectomy and treatment with 17?-estradiol did not alter the atria ER? mRNA expression,and the heart,ventricle ratio to body weight.Conclusion ERs expression is different between the newborn and adult rat heart,ER? may play an important role than ER? in the development of the rat heart,ER? high expression suggests that it functions as a predominant estrogenic mediator in adult heart.Estrogen mainly targets the atria;and activates atrio-myocyte proliferation via ER? mediated mechanism.
