IMMUNOHISTOCHEMICAL QUANTITY ANALYSIS OF THE SPLENIC LYMPHOCYTES IN THE PATIENTS WITH SEVERE ACUTE RESPIRATORY SYNDROME
- VernacularTitle:SARS患者脾淋巴细胞免疫组织化学的定量分析
- Author:
Jun ZHAN
;
Junmin TANG
- Publication Type:Journal Article
- Keywords:
Severe acute respiratory syndrome(SARS);
T cells;
B cells;
Immunohistochemical quantity analysis;
Spleen
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the distribution and the number of splenic lymphocytes in the patients with severe acute respiratory syndrome(SRAS).Methods Immunohistochemical method with four specific antibodies(CD3,CD4,CD8,CD20) was used to detect the distribution of lymphocyte special antigens in the spleens of six cases who died of SARS and six normal cases as the controls.The numbers of lymphocytes were analyzed with image analysis system.Results In the spleen of SARS patients,splenic corpuscle and periarterial lymphatic sheath (white pulp) were damaged severely. In the number of periarterial lymphatic sheath decreased 93.39% and in the number of splenic capsule decreased about 80% even disappeared at all. Red pulp hemorrhage necrosis was widely spread. In red pulp the number of CD3~(+)T cells decreased 71.76%,even disappeared at all,in the number of CD4~(+) and CD8~(+)T cells markedly decreased 86% and 84% respectively.The number of CD20~+ B cell decreased more than 80%. Conclusion T cells and B cells in spleens of SARS patients decreased widely spread and markedly in number,indicating that the immune system had been damaged severely,and the destroyment of the immunosystem may be the primary lesion of SARS.
