ULTRASTRUCTURAL STUDIES ON SPERMIOGENESIS OF TAKYDROMUS SEPTENTRIONALIS
- VernacularTitle:北草蜥精子形成的超微结构研究
- Author:
Yongzhang LIU
- Publication Type:Journal Article
- Keywords:
Spermiogenesis;
Ultrastructure;
Takydromus septentrionalis
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective We studied the ultrastructure of seprmiogenesis of Takydromus septentrionalis. Methods After double fixed with 2.5% glutaric dialdehyde and 1% osmic acid,we observed the paraffin section and ultrathin section by light microscope and transmission electron microscopy respectively. Results There is a series of considerable changes in morphology and structure during the spermiogenesis of Takydromus septentrionalis.These changes include gradual nuclear elongation,chromatin condensation,formation of acrosome,development and fusion of mitochondria,cytoplasmic sloughing and flagellar formation.The course of spermiogenesis can be devided into four stages:Stage Ⅰ,the spermatid nucleus concaved when the preacrosomal vesicle reached the nucleus,increasingly,acrosomal vesicle came into being and acrosomal granules appeared at the bottom of acrosomal vesicle,in a thin layer of granular cytoplasm.the subacrosomal granule was formed,it contacts both the acrosomal granule and the outer nuclear membrane;Stage Ⅱ,the spermatid acrosomal vesicle flattened and chromatin condensed into fibers like short filaments and the nucleus prolonged; Stage Ⅲ,the spermatid nucleus went on prologing and the chromatin fibers in it went on condensing and the chromatin fibers was put in a order of parallel to the longitudinal axis of the nucleus.The proximal centriole and flagellum appeared.Stage Ⅳ,the longitudinal microtubules in Sertoli cells cytoplasm appeared around the sperm head and disappeared till sperm was mature.The acrosomal complex of spermatid was well-developed,and annulus was appeared.The nuclear chromatin condensed to the greatest extent and the nucleus was electron-dense.Conclusion It has great significance of nucleus and cell organelle as taxonomic character of Squamate lizard in the ultrastructure of spermiogenesis of Takydromus septentrionalis.
