FLUORO-JADE B STAIN METHOD FOR DETECTING NEUROTOXICANT KAINIC ACID OR MPTP-INDUCED DEGENERATIVE DEATH OF NEURONS IN THE BASAL GANGLIA
- VernacularTitle:Fluoro-Jade B法显示神经毒物红藻氨酸和MPTP致小鼠基底神经核损伤引起神经元的变性死亡
- Author:
Rong CAO
;
Huijing HU
;
Yanqin WANG
;
Jinping ZHANG
;
Liangwei CHEN
- Publication Type:Journal Article
- Keywords:
Fluoro-Jade B;
Kainic acid;
MPTP;
Neuronal degeneration;
Basal ganglia;
Rat;
Mice
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To test experimentally Fluoro-Jade B(FJB) stain method for detecting degeneration of neurons in the basal ganglia. Methods Kainic acid(KA)-lesion model by stereotaxical injection of KA into striatum of rats,MPTP-lesion model by injection of MPTP into intraperitoneal cavity of mice,as well as KA-lesion model of cultured striatal cells were firstly prepared.FJB stain dye was then used to visualize degeneration of neurons in above KA-or MPTP-lesion models. Results KA-or MPTP-induced degenerative neurons including cell bodies and processes could be clearly visualized by FJB stain dye.In the brain sections,FJB-positive stained degenerative neurons were numerously observed in the striatum of KA-lesion rats and the substantia nigra pars compacta of MPTP-treated mice,but not detected in the control animals.Moreover,degenerative neurons were also detected with FJB stain in cultured striatal neurons.Semi-quantitative analysis on percentage(?s) of FJB-positive neurons constituting total cultured striatal neurons in unit area showed that degenerative neurons of KA-lesion group (8.42?1.09)% was evidently more than that of controls (3.42?0.45)%,P
