The effects of sodium hyaluronate on inducible nitric oxide synthase mRNA expression in cartilage of rabbit osteoarthritis
- VernacularTitle:透明质酸钠对兔骨关节炎软骨诱导型NO合酶基因表达的影响
- Author:
Yarong HAO
;
Bo QIU
;
Shiqing LIU
;
Lijun ZHANG
- Publication Type:Journal Article
- Keywords:
Osteoarthritis;
Sodium hyaluronate;
Nitric oxide synthase;
Cartilage
- From:
Chinese Journal of Rheumatology
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of intra-articular injection of sodium hyaluronate (Na-HA) on mRNA expression of inducible nitric oxide synthase (iNOS) in cartilage of osteoarthritis (OA). Methods Sixteen white rabbits were underwent unilateral anterior cruciate ligament transection and were divided into 2 groups randomly 5 weeks after transection. Experimental group rabbits received 0.3 ml of intra-articular 1% Na-HA injection once a week. Animals in control group were treated with the same conditions using normal saline. All animals were sacrificed 10 weeks following surgery. The aritcular cartilage degeneration of medial femoral condyle was evaluated at mascroscopically and microscopically. The mRNA expression of iNOS in cartilages was analyzed by reverse transcription-polymerase chain reaction (RT-PCR). Results Cartilage degradation in experimental group was significantly less severe than that in control group. No significant difference of iNOS mRNA expression in cartilage was found between the experimental group and the control group. Conclusion Na-HA can significantly reduce the severity of cartilage degradation of OA. Na-HA dose not show definite effect on iNOS expression in cartilage during early stage of OA.
