The study of relationship between the microsatellite polymorphism of cytotoxic T lymphocyte associated antigen-4 gene and inflammatory bowel disease
- VernacularTitle:细胞毒性T淋巴细胞相关抗原4基因微卫星多态性与炎症性肠病的关系
- Author:
Yi JIANG
;
Bing XIA
;
Junjie ZHENG
- Publication Type:Journal Article
- Keywords:
Cytotoxic T lymphocyte associated antigen-4;
Genetic polymorphism;
Inflammatory bowel disease
- From:
Chinese Journal of Digestion
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between the microsatellite polymorphism of cytotoxic T lymphocyte associated antigen-4 (CTLA-4) gene and inflammatory bowel disease(IBD) patients in Zhejiang province. Methods One hundred and eighteen IBD patients without consanguinity (99 patients with ulcerative colitis, 19 patients with Crohn's disease) and 140 healthy controls were studied. The (AT) repeats in the 3' untranslated region of exon 4 of CTLA-4 gene were amplified by allele-specific PCR. The amplified products were electrophoresed with 12% polyacrylamide gel, followed by silver staining. Results Twenty alleles were found in IBD patients and healthy controls. The frequency of 122 bp allele was higher in patients with ulcerative colitis (P=0.0001/Pc=0.0025, OR= 11.393, 95% CI:2.574-50.429) and Crohn's disease (P=0.0003/Pc=0.0050, OR=21.061, 95% CI:3.927-112.94) than that in healthy controls. Conclusion CTLA-4 gene microsatellite polymorphism was obviously associated with IBD in Zhejiang province.
