The effect of exogenous Smad7 gene on activation of hepatic stallete cell in primary culture and its role in the regulation of gene expression
- VernacularTitle:外源Smad7基因对原代肝星状细胞活性和基因表达调控的影响
- Author:
Yingwei CHEN
;
Yuanwen CHEN
;
Hanning YOU
- Publication Type:Journal Article
- Keywords:
Adenovirus vector;
Hepatic stellate cell;
Transforming growth factor
- From:
Chinese Journal of Digestion
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Smad7 gene on the activation of rat hepatic stellate cells (HSCs) in primary culture and on the regulation on gene expression of HSCs. Methods HSCs were isolated from male SD rats by collagenase perfusion of liver from portal vein and by 8.2% Nycodenz gradient centrifugation, and thereby transfected with AdSmad7 and AdGFP (control) respectively. The mRNA expression of transforming growth factor (TGF) ?1, Smad3 and Smad7 were measured by RT-PCR. Meanwhile, Smad7 and ?-smooth muscle actin (?-SMA) expressions were detected by immuno- cytochemistry. Results The expression of Smad7 mRNA in AdSmad7 group increased remarkably compared with TGF?1 control group, while the expression of Smad3 and TGF?1 mRNA remains unchanged. The expression of Smad7 protein was significantly higher in AdSmad7 group than that in other three groups. Accordingly, the expression of ?-SMA protein in the group of HSCs transfected with AdSmad7 was the lowest(P
