Inductive effect of Prunella vulgaris Injection on K562 cells apoptosis
- VernacularTitle:夏枯草注射液诱导K562细胞凋亡的实验研究
- Author:
Kejie ZHANG
;
Mingzhi ZHANG
;
Qingduan WANG
- Publication Type:Journal Article
- Keywords:
Prunella vulgaris Injection(PVI);
K562 cells;
apoptosis;
apoptosis related protein
- From:
Chinese Traditional and Herbal Drugs
1994;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the antileukemia effect of Prunella vulgaris Injection(PVI) in order to offer experimental data for the treatment of leukemias with PVI in clinic.Methods Inhibition of PVI on K562 cell proliferation and IC_(50) value was assayed by MTT assay.The growth curve of K562 cells was drawn also.The cellular morphology was observed by invert microscope,Giemsa staining,and MTT assay.The apoptosis induced the effect of PVI to K562 cells was observed by trypan-blue exclusion test and flow cytometer(FCM).The expression of apoptosis related protein bcl-2 and bax was measured by immunocytochemistry,and the quantitative analysis was made with figure analysis system.Results PVI obviously inhibited the proliferation of K562 cells in a dose-dependent manner(r=0.985 0).The IC_(50) was 0.113 mg/mL.(After) K562 cells were treated with PVI(50 mg/mL),the morphological characters of apoptosis were observed.The results of FCM showed that the apoptosis rats [(37.15?1.46)%] in the treatment group were significant higher than that in control group [(5.56?0.68)%](P
