Construction of LAPTM4B promoter-luciferase reporter recombinants
- VernacularTitle:LAPTM4B启动子荧光素酶报告基因重组体的构建
- Author:
Fangyi PENG
- Publication Type:Journal Article
- Keywords:
LAPTM4B (lysosome associate protein transmembrane 4 beta);
Gene expression;
Promoter
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:LAPTM4B is a novel gene which is over expressed in HCC.To evaluate LAPTM4B gene promoter on gene transcription,we construct luciferase reporter plasmid pGL3-LAPTM4B-Luc.Methods:The LAPTM4B cDNA was used as a template in the polymerase chain reaction to amplify its upstream regions from the translation initiation codon.The PCR product was directly cloned into the luciferase reporter vector pGL3-Basic.Results:Restriction enzymes digestion and nucleotide sequencing confirmed that the recombinant plasmids were correct without base mutation and deletion.Conclusion:A known gene promoter sequence could be freely obtained from Genbank database.This is very useful for the gene promoter cloning and promoter vector constructing.The construction of LAPTM4B recombinant plasmid lay a foundation of the analysis of promoter activities.
