Inhibition of proliferation of human hepatocellular carcinoma cell lines by TNBG in vitro
- VernacularTitle:德氮吡格对人肝癌细胞株增殖抑制的体外研究
- Author:
Longjiang LI
- Publication Type:Journal Article
- Keywords:
SMMC-7721, HepG2;
CyelinB1;
Cell cycle;
Inhibition of proliferation
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of TNBG on proliferation of human hepatocellular carcinoma cell lines SMMC-7721 and HepG2.Methods:MTT assay was used to test the effects of anti-proliferation.The flow cytometric analysis was used to detect the cell cycle distribution.Western blot was adopted for detecting the expression of cyclinB1 and p34 cdc2 .Results:Anti-proliferation effects were observed in SMMC-7721 and HepG2 after intervention of TNBG for 72 hours,and the effects were in concentration-dependent manners.The cells showed S and G2/M arrest in both of cell lines,and apoptosis was induced only in HepG2.The expression of cyclinB1 was inhibited,while p34 cdc2 was not changed.Conclusion:TNBG can inhibit the proliferation of tumor cells,which is due to G2/M arrest caused by expression inhibition of cyclinB1 induced by TNBG.
