Activation of MAPK~(ERK1/2) signal transduction pathway in the hippocampal formation of rat after experimental seizure
- VernacularTitle:MAPK~(ERK1/2)信号转导途径在实验性癫痫大鼠海马结构的激活
- Author:
Jie XIA
- Publication Type:Journal Article
- Keywords:
ERK;
Epilepsy;
Hippocampal formation;
Pilocarpine
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the spatiotemporal distribution pattern of active ERK1/2 in rat hippocampal formation following pilocarpine-induced status epilepticus(SE) and to discuss its significance.Methods:The lithium-pilocarpine-induced model of SE was established to evaluate the phosphorylation forms of ERK1/2(p-ERK1/2) by immunohistochemistry and observe histopathology changes at different times after pilocarpine injection.Results:The p-ERK1/2 immunoreactivity was observed only in neurites of saline-treated rats hippocampal formation.In pilocarpine-treated rats,the hippocampal neurons showed a strong immunoreactivity of p-ERK1/2 in the body at 30 min which peaked at 2h after pilocarpine injection,while decreased markedly at 6h and returned to basal level at 12h.There were more immunoreactive positive cells in dentate gyrus than those in regions of CA1 and CA3.Many injured neurons were found in hippocampus of SE rats.Conclusion:MAPK ERK1/2 signal transduction pathway can be activated in SE induced by pilocarpine,and may play a critical role in the pathophysiological process of neuron injury and protection related to seizure,and even mediate further long-lasting morphological changes.
