PPAR? activators inhibitory effects on angiotensinⅡ induced tumor necrosis factora expression in neonatal rat cardiac myocytes
- VernacularTitle:PPAR?激动剂抑制AngⅡ诱导乳鼠心肌细胞合成肿瘤坏死因子
- Author:
Hualing WANG
- Publication Type:Journal Article
- Keywords:
Peroxisome proliferator-activated receptor ?;
Tumor necrosis factor ?;
Angiotensin Ⅱ;
Cardiac myocyte
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of the nuclear receptor peroxisome proliferator-activated receptor (PPAR)? activators on tumor necrosis factor ? (TNF-?) expression in neonatal rat cardiac myocytes.Methods:Primary culture of cardiac myocytes was prepared from 1～3 days old Spraque-Dawley rats.Cardiac myocytes were pretreated with rosiglitazone at different concentrations and then stimulated with angiotensin Ⅱ(1 ?mol/L).ELISA and reverse transcripition-polymerase chain reaction(RT-PCR) were used to examine TNF-? in cultured supernatants and TNF-? mRNA in cardiac myocytes,respectively.Results:Pretreatment of cardiac myocytes with rosiglitazone inhibited the increase of TNF-? and TNF-? mRNA induced by angiotensinⅡ in a concentration-dependent manner.Conclusion:Rosiglitazone inhibits the expression of TNF-? in cardiac myocytes,which provides a new way for treating chronic heart failure.
