The construction and significance of maspin/PCR2.1 expression vector
- VernacularTitle:maspin/PCR2.1表达载体的构建及意义
- Author:
Xiong YAN
- Publication Type:Journal Article
- Keywords:
Maspin;
PCR2.1 expression vector;
Construction;
Significance
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct the expression vector maspin /Pcr2.1 in order to provide the evidence for the inhibitory effect of maspin gene on growth,metastasis and invasion of hepatocellular carcinoma(HCC) and to discuss its mechnism.Methods:The full length of maspin gene was cloned,and the expression vector PCR2.1 was digested by Hind111/XbaI,then the maspin gene and the PCR2.1 vector were ligated with T4 DNA ligase to be recombinant vector.The recombinant vector maspin/PCR2.1 was transfected into humman hcc MHCC-97 cell line,and the expressive changes of maspin gene were detected.Results:The recombinant plasmid was amplified in the E.coli.JM109.After the identification of redigesting by HindIII/XbaI and sequencing,the reconstructive plasmid was confirmed to contain the correct and full nucleotide sequence of maspin gene,and the mRNA and protein level of maspin gene were up-regulated in hcc MHCC-97 cell.Conclusion:The expression vector maspin/PCR2.1 is constructed successfully and can be expressed in eukaryotic cells.
