Effects of mycobacterium vaccae vaccine on eosinophil apoptosis,protein expression of Fas and bcl-2 in lung tissues of asthmatic guinea pig
- VernacularTitle:母牛分枝菌菌苗对哮喘豚鼠肺组织嗜酸粒细胞凋亡及Fas、bcl-2蛋白表达的影响
- Author:
Hong CHEN
- Publication Type:Journal Article
- Keywords:
Mycobacterium vaccae vaccine;
Asthma;
Eosinophil;
Apoptosis;
Fas;
bcl-2
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of Mycobacterium vaccae vaccine on eosinophil apoptosis,protein expression of Fas and bcl-2 in lung tissues of asthmatic guinea pig.Methods:30 guinea pigs were randomly divided into NS,asthma and Mycobacterium vaccae vaccine group.A guinea pig model of asthma was estbalished with OVA(ovalbumin).Each guinea pig of Mycobacterium vaccae vaccine group was injected intramuscularly with 22.5?g of Mycobacterium vaccae vaccine 10 days before OVA immunization.After the last challenge,TdT-mediated dUTP nick end labeling (TUNEL)technique was used to determine the apoptosis of eosinophils and immunohistochemistry method was used to determine protein expression of Fas and bcl-2 in lung tissue sections.Results:Guinea pigs of Mycobacterium vaccae vaccine group had a higher AI(apoptosis index) of eosinophils in lung tissues[(23.8?5.4)%]than that of asthma group[(4.6?0.7)%,P
