The effect of exogenous hyaluronidase on proliferation of human breast cancer cells in vitro
- VernacularTitle:外源性透明质酸酶对人乳癌细胞株体外增殖的影响
- Author:
Liang WANG
- Publication Type:Journal Article
- Keywords:
Hyaluronidase;
Breast cancer;
Cell cycle
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of exogenous hyaluronidase on proliferation of human breast cancer cells in vitro.Methods:The proliferation of human breast cancer cell line ZR-75-30 were detected by MTT-assay and flow cytometry.Results:The absorbency value(A) of ZR-75-30 in study group treated with Hyase (0.674?0.221) was significantly higher than that in control group(0.563?0.046).The percentage of phase S cells in study group(18.36?0.65) was higher than that in control group(2.19?0.10);the percentage of phase G0/G1 cells in study group(44.49?4.33) was lower than that in control group(62.14?26.97).The absorbency value(A) of MDA-MB-435 in study group (0.674?0.221) was significantly higher than that in control group(0.563?0.046).The absorbency valve(A) of MDA-MB-231 in study group (0.674?0.221) was significantly higher than that in control group(0.563?0.046).Conclusion:Exogenous hyaluronidase may promote the proliferation of breast cancer cell lines in vitro.
