Effects of Plastrum Testudinis on Os teogenesis of Cultured Rat Mesenchymal Stem Cells
- VernacularTitle:龟板对大鼠骨髓间充质干细胞向成骨分化的影响
- Author:
Hui LI
;
Jianhong ZHOU
;
Dongfeng CHEN
;
Shaohui DU
;
Yiwei LI
;
Rudong DENG
;
Saixi ZHANG
- Publication Type:Journal Article
- Keywords:
Plastrum Testudinis;
Mesenchymal stem cells;
Osteoblast;
Pharmacology
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of kidney-tonifying Plastrum Testudinis on o steogenesis of cultured rat mesenchymal stem cells(MSCs ).Methods MSCs were isolated from adult rats wi th density gradient separation meth od.Osteogenesis of MSCs in the culture a dded with different concentrations of serum containing Plastrum Testudinis was evalu-ated by detecting bone glaprotein(BGP)level and observing the morphologic al features of MSCs and by alkaline ph os-phatase(ALP)and Von Kossa immunohistochemical m ethods.Results Morphological examination showed t hat the MSCs attachment formed soon after seedin g ,and grew into colonies with the appearance of fibroblastic cells.Seru m containing Plastrum Testudinis promoted the expression of alkaline phosphatase(ALP)in MSCs ,and increased BGP level and the number of calcified deposition in dose -dependant manner,the difference being significant in comparison wi th the control groups(P
