Determination of Berberine Content in Qixue Guben Oral Liquid by TLCS
- VernacularTitle:薄层扫描法测定气血固本口服液中小檗碱的含量
- Author:
Shuling WANG
;
Cheng CAO
- Publication Type:Journal Article
- Keywords:
Qixue Guben oral liquid;
Berberine;
Content determination;
Thin layer chromatography scanning
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a new method for the determ ination of berberine in Qixue Guben oral liquid(QGOL).Method Double wavelength thin -layer chrom atography scanning(TLCS)was performed with benzene -ethyl ac-etate -methanol -isopropyl alcohol-water(6∶3∶1.5∶1.5∶0.3)as developer,detection wavelength at 345nm and reference wavelength at 370nm.Results The berberine hydrochloride could b e separated from QGOL.The calibration curve was linear in the range of 0.242?g ～1.210?g for berberine.The average recovery was 97.1%and RSD was 2.4%(n=5).Conclusion This method is simple,accurate and c an be used for the quality control of Q GOL.
