Fluorimetric Determination of the Complex of Tetramethylpyrazine and Human Serum Albumin
- VernacularTitle:川芎嗪与人血清白蛋白结合的荧光法测定
- Author:
Xin HONG
;
Suiqing MI
;
Zhiyong XU
- Publication Type:Journal Article
- Keywords:
Tetramethylpyrazine;
Human serum albumin;
Fluorescence;
Interaction
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the interaction of tetramethylpyrazine and human serum albumin(HSA).Methods The fluorescent quenching of HSA with te tramethylpyrazine has been detecte d by fluorimetry.Results It was found that fluo-rescence of HSA were quenched by tetr amethylpyrazine.The fluorescent q uenching data were analyzed accordi ng to Stern -volmer equation and the bindi ng constant was obtained.Conclusion The mechanism of fluorescent quench ing is considered to be the formation of HSA-tetramethylpyrazine complex.
