Determination of baicalin and emodin in Yiqing Capsule by HPLC
- VernacularTitle:HPLC双波长法测定一清胶囊中黄芩苷、大黄素的含量
- Author:
Kewei ZHANG
;
Wei LI
;
Hao CAI
;
Xiaobing CUI
- Publication Type:Journal Article
- Keywords:
HPLC;
Yiqing Capsule;
dual wavelength;
baicalin;
emodin
- From:
Chinese Traditional Patent Medicine
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a HPLC for determination of baicalin and emodin in Yiqing Capsule(Radix Scutellarial, Radix et Rhizoma Rhei). METHODS:A Kromasil C_(18) Column was used. The mobile phase was methanol -0.2% phosphoric acid for gradient elution. Baicalin and emodin were determined by dual wavelength, ?_1=315 nm, ?_2=266 nm. RESULTS:The linear range of baicalin was within 64.40-322.00 ?g?mL~(-1) (r=(0.999 8)). The average recovery was 99.96%,RSD was 1.57%(n=9). The linear range of emodin was within( 0.82)-13.20 ?g?mL~(-1)(r=0.999 9).The average revovery was 98.90%, RSD was 1.25%(n=9). CONCLUSION: This method has good repeatability and flexibility. It can be used for quality control in production of Yiqing Capsule. (Nan
