Determination of astragloside Ⅳ in Danqi Injection by HPLC-ELSD
- VernacularTitle:HPLC-ELSD测定丹芪注射液中黄芪甲苷的含量
- Author:
Guiyou WANG
;
Haijun CHEN
;
Ziyu ZHAO
;
Weiqin JI
;
Jinbao DONG
- Publication Type:Journal Article
- Keywords:
Danqi Injection;
HPLC;
ELSD;
astragloside Ⅳ
- From:
Chinese Traditional Patent Medicine
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a method for determination of astragloside Ⅳ in Danqi Injection (Radix Salviae Miltiorrhizae,Radix Astragali). METHODS:HPLC-ELSD with Hypersil C_(18) column was used.The mobile phase was composed of acetonitrile-water(38∶62).The flow rate was 0.85 mL?min~(-1).An evaporative light-scattering detector(ELSD) model 2000 was used as detector. RESULTS:For astragloside Ⅳ,the linear range was 1.074-(3.408 ?g),and the average recovery was 100.71%,RSD was 1.79%. CONCLUSION:The method is simple,accurate,reproducible and can be applied to the quality control of the preparation. anqi
