Expression of vascular endothelial growth factor and its receptors mRNA after focal cerebral ischemic-reperfusion in rats
- VernacularTitle:大鼠脑缺血再灌注损伤后VEGF、Flt-1、Flk-1 mRNA的表达及意义
- Author:
Chanjuan ZHENG
;
Weijing LIAO
;
Wantong YANG
;
Lanqing MENG
- Publication Type:Journal Article
- Keywords:
Cerebral ischemia-reperfusion;
VEGF;
Receptor
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of vascular endothelial growth factor (VEGF) and its receptors(VEGFR) mRNA at different time points after focal cerebral ischemic-reperfusion(CIR) in rats. Methods Following the establishment of the of transient ischemia modelof middle cerebral artery in rats by Zea-Longa′s method, the expression of VEGF and its receptors mRNA was measured with semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) at different time points. Results The expression of VEGF mRNA increased 3 h after CIR,and peaked at 6 h, and then declined, returned to the baseline at 7 d. The expression of Flt-1 and Flk-1 mRNA was enhanced at 3 h, and reached its peak at 3 d, then declined gradually. Conclusion The expression of VEGF、Flt-1 and Flk-1 mRNA can be induced by focal CIR in rats.
