Inhibitory effects of antisense Snail on invasion and migration of ovarian carcinoma cells
- VernacularTitle:反义Snail转录因子抑制卵巢癌转移的实验研究
- Author:
Hongyan JIN
;
Youji FENG
- Publication Type:Journal Article
- Keywords:
Ovarian neoplasms;
Transcription factors;
Cadherins;
Transfection;
Neoplasm metastasis
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effects of Snail—— a zinc finger transcription factor, anti-sense plasmid on the invasion of ovary carcinoma cell lines in vitro. Methods Four human ovarian carcinoma cell lines ES-2,OVCAR3,HO8910,HO8910PM were analyzed for the expression of Snail and E-cadherin mRNA by RT-PCR.Then anti-Snail plasmid transfection was introduced into HO8910 cells using lipofectin 2000 reagent to investigate the inverse correlation between E-cadherin and Snail expression, and the inhibitory effects of anti-sense Snail were also detected by Transwell motility assay and Matrigel invasion assay. Results The results demonstrated the presence of Snail mRNA in ES-2,HO8910,HO8910PM detected by RT-PCR. By contrast, E-cadherin mRNA was only detected in OVCAR3 by RT-PCR.The inverse relationships were further observed by transient transfection of anti-sense Snail into HO8910 cells, which showed the down-regulated expression of Snail and the re-expression of E-cadherin. The Snail mRNA levels were 0.897?0.005,0.865?0.010,0.338?0.014 after 0,24,48 h of transfection and that of E-cadherin were 0,0.130?0.001,0.217?0.005 respectively, the difference was significant between different time points (P
