Determination of triptoquinone H in Tripterygium wilfordii and its Tablet by HPLC
- VernacularTitle:HPLC法测定雷公藤药材及其片剂中雷公藤对醌H
- Author:
Jiangping HE
;
Wenyan WANG
;
Wenyuan GAO
;
Yanwen ZHANG
;
Wen ZHOU
;
Hongquan DUAN
- Publication Type:Journal Article
- Keywords:
Tripterygium wilfordii Hook. f.;
tablet;
triptoquinone H;
HPLC
- From:
Chinese Traditional and Herbal Drugs
1994;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an HPLC method for determination of triptoquinone H in Tripterygium wilfordii and its Tablet preparations. Methods An external method with Agilent Zorbax SC-C_(8)(250 mm?4.6 mm, 5 ?m) column as fixed phase and methanol-water (75∶25) as mobile phase was adopted. The detective wavelength was 258 nm and the flow rate was 1.0 mL/min. Results The linearity range of triptoquinone was 6.28 — 100.5 ?g/mL (r=0.999 7). The average recovery of T. wilfordii was 96.94%, RSD was 1.57% (n=9) and the average recovery of T. hypoglaucum Tablet was 100.02%, RSD was 1.74% (n=9). Conclusion The method is accurate and sensitive. It is adoptable for quantity analysis of triptoquinone H in T. wilfordii and its Tablet.
