Effect of Sailong bone extracts on rat osteoblastoid cells ROS17/2.8 in vitro
- VernacularTitle:塞隆骨提取物对体外大鼠成骨样细胞ROS17/2.8的影响
- Author:
Ming PAN
- Publication Type:Journal Article
- Keywords:
Sailong bone;
osteoblastoid cells;
proliferation;
differentiation
- From:
Chinese Traditional and Herbal Drugs
1994;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the modulation of Sailong bone extracts on rat osteoblastoid cells ROS17/2.8 in vitro so as to clarify the mechanism of osteoporosis prevention and active component screening of Salong bone. Methods Sailong bone fat-soluble extract, Sailong bone ethanol extract and Sailong bone aqueous extract were extracted with super critical fluid extraction (SCFE), and Sailong bone boiling-water component was extracted with distilled water directly. MTT assay was applied to determine the proliferation of the cell promoted by four Sailong bone extracts at different doses. Differentiating effects of the extracts with different concentrations on the cell were evaluated by the examinations of alkali phosphate (ALP) activity. Results Sailong bone fat-soluble and aqueous extract (each 10 mg/mL) could significantly improve the proliferation of rat osteoblastoid cells ROS17/2.8 (P
