THE EFFECT OF THE EXPERIMENTAL LEAD EXPOSURE ON EXPRESSION OF NERVE GROWTH FACTOR GENE IN HIPPOCAMPUS OF RAT
- VernacularTitle:实验性铅中毒对大鼠海马神经生长因子基因表达的影响
- Author:
Kaifu KE
;
Mi SHEN
;
Fei DING
- Publication Type:Journal Article
- Keywords:
Nerve growth factor;
Lead;
Hippocampus;
RT-PCR;
In situ hybridization;
Rat
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of lead exposure on expression of nerve growth factor(NGF)gene in the rat hippocampus,and explore the molecular toxicological mechanism of the learning and damage induced by lead exposure. Methods Using the reverse transcription-polymerase chain reaction(RT-PCR)and in situ hybridization,NGF mRNA levels in the hippocampus of normal and lead exposure rats were investigated. Results NGF mRNA presented in the normal hippocampus of rats.NGF mRNA levels in the hippocampus were significantly decreased by lead exposure.There was a negative correlativity between the expression of nerve growth factor mRNA in the hippocampus and the time of lead exposure.Conclusion Lead can downregulate the expression of nerve growth factor gene in the rat hippocampus.
