THE MORPHOLOGICAL OBSERVATION OF THE OSTEOBLAST CULTURED WITH RECONSTRUCTED ACELLULAR EXTRACELLULAR MATRIX IN VITRO
- VernacularTitle:重组骨脱细胞细外基复合Wistar大鼠成骨细胞体外培养的形态学观察
- Author:
Wei TIAN
;
Changqing JIA
;
Shulin BAI
- Publication Type:Journal Article
- Keywords:
Bony tissue engineering;
Osteoblasts;
Bony reconstructed acellular extracellular matrix;
Co-culture;
Rat
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The construction of the reconstructed acellular extracellular matrix(REAECM) of bone and preliminary investigation of biocompatibility of REAECM was performed to supply the experimental basis for the extracellular scaffold of bone tissue engineering. Methods Applying the technique of culture in vitro, biocompatibility of the REAECM cultured with osteoblasts was observed using phase-contrast microscope and scanning eletron microscope at 1-4 weeks. Results The osteoblasts could attach and proliferate, even grow well into the foam of REAECM.Conclusion REAECM is an excellent scaffold material for bony tissue engineering construction, which pocesses a morphological structure of three-dimensional foam. The REAECM showed a good biocompatibility with osteoblasts co-cultured in vitro. There was prospective signs of REAECM to the attachment, proliferation of the osteoblasts
