Variation of virulence in galE knock-out mutant of Campylobacter jejuni
- VernacularTitle:galE基因敲除后空肠弯曲菌变异株毒力的改变
- Author:
Xiaomei SHU
- Publication Type:Journal Article
- Keywords:
Campylobacter jejuni;
Mutant;
Virulence;
Invasion;
Adhesion
- From:
Journal of Chongqing Medical University
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the change in virulence of Campylobacter jejuni (CJ) mutant after galE gene knock-out.Methods: Using Hep-2 cells, adherence and invasion assays were performed in the galE mutant and the parent strain, meanwhile, serum sensitivity assay and motility test were made in them. Results:The galE mutant showed a reduction in its ability to adhere to and invade Hep-2 cells. The ability of the galE mutant and parent strain to adhere to Hep-2 cells were 1.2 % and 8.3% respectively, and adherence ability of the former only was 14.5% of that of the latter. The difference was significant ( P
