Investigation methods of diagnosis of Chlamydia trachomatis by Gap ligase chain reaction
- VernacularTitle:缺口-连接酶链反应-酶联免疫吸附(Gap-LCR-ELISA)检测沙眼衣原体的方法学研究
- Author:
Chun DENG
- Publication Type:Journal Article
- Keywords:
Chlamydia trachomatis;
Ligase-chain reaction;
Nasopharyngeal swab
- From:
Journal of Chongqing Medical University
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the method with good sensitivity,specificity,reliability for diagnosis of Chlamydia trachomatis.Methods:Inoculate the nasopharyngeal swabs to detect the Chlamydia trachomatis(CT) with McCoy cell culture and plasmid gene probes labeled with biotin ligase-chain reaction.Then calculate the sensitivity,specificity,positive predicative value(PPV) and negative predicative value(NPV) of both TC and G-LCR respectively.Compare the difference of the two methods.Results:There were 49 positive specimens and 344 negative by enlarged gold standard.There was significant difference with cell culture,G-LCR-PAGE,G-LCR-ELISA by two-related-samples ? 2 tests( P
