A role of sodium selenite in regulating expression of p38 mitogen-activated protein kinase (p38MAPK) and vascular endothelial growth factor (VEGF)in rat mesangial cells
- VernacularTitle:亚硒酸钠对大鼠肾小球系膜细胞p38MAPK和VEGF表达的调控
- Author:
Qianping WEI
- Publication Type:Journal Article
- Keywords:
Sodium selenite;
P38 mitogen-activated protein kinase;
Vascular endothelial growth factor;
Diabetic nephropathy;
Rat mesangial cells
- From:
Journal of Chongqing Medical University
1986;0(02):-
- CountryChina
- Language:Chinese
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Abstract:
Objective:To investigate the relationship between p38 mitogen-activated protein kinase(p38MAPK) and vascular endothelial growth factor (VEGF),and to study the role of p38 mitogen-activated protein kinase and vascular endothelial growth factor for diabetic nephropathy,and therefore to study the mechanism of sodium selenite in anti-diabetic nephropathy.Methods:We initially investigated protein expression of p38MAPK and VEGF in rat mesangial cells(RMCs)which were incubated respectively with 25 mmol/L glucose,100mg/L BSA-AGEs,100nmol/L insulin and 100 ?mol/L H 2O 2.We also studied the relationship between p38MAPK and VEGF protein expression by using SB203580,a specific inhibitor of p38MAPK.We then evaluated the direct effects of sodium selenite on the regulation of p38MAPK and VEGF protein expression.Results:p38MAPK and VEGF had significantly higher expression in RMCs incubated with 25mmol/L glucose,100mg/L BSA-AGEs,100nmol/L insulin and 100?mol/L H 2O 2 respectively.VEGF activity was significantly reduced when p38MAPK was inhibited by SB203580.Furthermore,both p38MAPK and VEGF protein expression were significantly decreased in RMCs with sodium selenite treatment.Conclusion:p38MAPK and VEGF are involved in development of diabetic nephropathy and p38MAPK stimulation is essential for VEGF expression,and also suggests that sodium selenite may play a role in the prevention of diabetic nephropathy by down-regulation of both p38MAPK and VEGF expression.
