The tffects of two tyrosine kinase inhibitors on the proliferation of colon cancer cell
- VernacularTitle:两种酪氨酸激酶抑制剂对结肠癌细胞增殖的影响
- Author:
Yujuan ZHU
- Publication Type:Journal Article
- Keywords:
Colon cancer;
Extralcellular-signal regulated kinase;
Tyrosine kinase inhibitors
- From:
Journal of Chongqing Medical University
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of Genistein and Tyrphostin AG1478 on the proliferation of colon cancer cell HT-29 and its possible mechanism.Methods:The effect of two tyrosine kinase inhibitors (TKIs) on HT-29 cell lines was studied by using MTT assay.Immunocytochemical staining was performed with PCNA polyclonal antibody in HT-29 cells.Western blot assay was used to detect the expression of ERK-1 and pERK.Results:Both Genistein and Tyrphostin AG1478 significantly inhibit the growth of HT-29 cells and showed concentration dependence.Two tyrosine kinase inhibitors suppressed PCNA expression.Western blot revealed that Genistein and Tyrphostin not inhibit ERK-1 expression but inhibit phosphorylation of ERK.Conclusion:Tyrosine kinase inhibitors Genistein and Tyrphostin AG1478 can inhibit the growth of HT-29 colon cancer cells,the anti-proliferation mechanism may be related to down-regulate the expression of pERK.
