Effect of silencing mdrl gene by RNA interference with adriamycin on proliferation of SKOV_3/ADM
- VernacularTitle:RNA干扰沉默mdrl基因联合阿霉素对卵巢癌耐药细胞株SKOV_3/ADM增殖的抑制作用
- Author:
Pingling WANG
- Publication Type:Journal Article
- Keywords:
Ovarian cancer;
Multidrug resistance (MDR);
RNAi;
mdrl gene
- From:
Journal of Chongqing Medical University
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of RNA interference targeting mdrl gene with ADM on ovarian cancer resistant strain SKOV 3/ADM.Methods:The expression of mdrlmRNA after transfected pshRNA-mdrl was detected by RT-PCR;MTT test and clone forming test were applied to measure the inhibition combined with adriamycin on proliferation of SKOV 3/ADM strain.Results:These pshRNA-mdrls inhibited the expression of mdrlmRNA and proliferation of SKOV 3/ADM obviously when combined with adriamycin.Conclusion:The short hairpin RNA recombinant plasmid targeting mdrl gene combined with adriamycin inhibits the proliferation of SKOV 3/ADM and increases the sensitivity to chemotherapy.
